Johannes Krug, Birgit Perner ... Christoph Englert
Using the short-lived turquoise killifish, a transparent in vivo reporter line named klara is established that will be a valuable tool to explore development, senescence, aging, and regeneration.
Rapid and efficient CRISPR/Cas9-mediated knock-in of fluorescent reporters at various genomic loci enables cell- and tissue-specific expression and establishes the short-lived African killifish as a vertebrate system for precise genetic engineering at scale.
A novel in vivo strategy for reversible, multiplexed control of circulating hormone expression levels, achieved by combining genome-editing and intramuscular electroporation of fluorescently tagged hormones in the naturally short-lived turquoise killifish.
An automated feeding system to precisely control feeding in the killifish opens new areas to explore vertebrate lifespan, cognitive decline, dietary interventions, and drug screening in a high-throughput manner.
The turquoise killifish from ephemeral pools in African savannah combines extremely short lifespan with a standard vertebrate body plan – ideal attributes for a laboratory animal.
David Willemsen, Rongfeng Cui ... Dario Riccardo Valenzano
Population genetics in turquoise killifish wild populations reveals how small population size and genetic drift determine the accumulation of deleterious gene variants leading to short lifespan.
