Roberta Cacioppo, Hesna Begum Akman ... Catherine Lindon
Post-transcriptional regulation of Aurora Kinase A (AURKA) through alternative polyadenylation of its mRNA determines miRNA influence and is a feature of cell cycle-specific AURKA expression whose impairment leads to acquisition of cancer phenotypes.
Distinct interactions of AP endonuclease 1 with single-stranded DNA gaps and ATRIP and RPA protein complex address an outstanding question in the field of genome integrity regarding how ATR/ATRIP complex recruits onto damage site for ATR DNA damage response signaling.
The anti-tumor action of engineered mesenchymal stem cells highlights the double-edged role of oncoproteins in osteosarcoma, and suggests the possibility of developing a novel strategy for protein-based cancer therapy.
The development of an integrative computational-experimental approach for identifying small molecules that can disrupt RNA:protein interactions in vitro and in cells led to the identification of several inhibitors of YBX1 in the micromolar range, including a previously approved drug.
SIMC1 and SLF1 bind exclusively to SLF2 to form two separate complexes that direct the human SMC5/6 complex to its antiviral defense or DNA lesion repair activities.
A sophisticated classical mutagenesis assay with next-generation sequencing technology presents a benefit to future research on mutagenesis, DNA repair, and cancer.
Tolulope Sokoya, Jan Parolek ... Joost CM Holthuis
Organellar lipidomics and lipid reporter studies in intact cells reveal how disease-relevant mutations in a sphingolipid biosynthetic enzyme cause a wide-ranging perturbation of lipid distributions and membrane properties along the secretory pathway.
Organoids developed from matched human placental tissue define differences in antiviral signaling between cell types comprising the maternal-fetal interface.
Tracking proteins in live cells is challenging due to technical limitations and biological complexity, but approaches based in Bayesian nonparametrics stand a decent chance at recovering a target protein's dynamic profile from noisy data.
