Topoisomerase 2α DNA breaks induced by G-quadruplex ligands are associated with a topological stress resulting from a transcription-dependent mechanism and counteracted by DNA topoisomerase 1 and factors promoting transcription elongation.
A high-fidelity and efficient strategy to use CRISPR/Cas9 to reversibly insert large DNA fragments into somatic cells to label or modify endogenous proteins for research and, in the future, for gene therapy.
Monitoring SHP2 phosphoproteome dynamics identifies new substrate sites and sites protected from dephosphorylation by its SH2 domains, highlighting distinct scaffolding and catalytic activities in effecting a transmembrane signaling response.
The lamin A/C binding protein LAP2α inhibits formation of higher order lamin structures in the nuclear interior in a lamin A/C-phosphorylation-independent manner, thereby regulating chromatin mobility.
Optogenetic experiments show that bridging microtubules buffer chromosome movements and promote their alignment through forces transferred to the associated kinetochore fibers, which rely on precise regulation of the overlap region.