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    1. Structural Biology and Molecular Biophysics

    Enhanced specificity mutations perturb allosteric signaling in CRISPR-Cas9

    Lukasz Nierzwicki, Kyle W East ... Giulia Palermo
    Allostery holds critical role in CRISPR-Cas9 specificity enhancement.
    1. Genetics and Genomics

    Fast turnover of genome transcription across evolutionary time exposes entire non-coding DNA to de novo gene emergence

    Rafik Neme, Diethard Tautz
    Deep sequencing of transcriptomes from closely related taxa shows that the whole genome can become subject to evolutionary testing within a short time frame via de novo evolution of genes.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Contributions of mast cells and vasoactive products, leukotrienes and chymase, to dengue virus-induced vascular leakage

    Ashley L St John, Abhay PS Rathore ... Soman N Abraham
    Proteins produced by mast cells modulate the permeability of blood vessels, and may determine whether patients infected with dengue virus develop life-threatening complications.
    1. Cancer Biology
    2. Genetics and Genomics

    Patient-derived xenografts and single-cell sequencing identifies three subtypes of tumor-reactive lymphocytes in uveal melanoma metastases

    Joakim W Karlsson, Vasu R Sah ... Jonas A Nilsson
    Single-cell sequencing and functional analysis identify T cells that can be useful for marker selection and cell therapy in uveal melanoma, a disease largely unresponsive to conventional immune checkpoint therapies.
    1. Computational and Systems Biology

    Response to comment on ‘SARS-CoV-2 suppresses anticoagulant and fibrinolytic gene expression in the lung’

    Alan E Mast, Alisa S Wolberg ... Daniel Jacobson
    We are writing to respond to the comment by FitzGerald and Jamieson, 2022 on our article about the drivers of coagulopathy in the lungs of COVID-19 patients (Mast et al., 2021).
    1. Neuroscience

    Jointly looking to the past and the future in visual working memory

    Baiwei Liu, Zampeta-Sofia Alexopoulou, Freek van Ede
    When memorising dynamic visual objects, the brain codes for both the past and the anticipated future object location and co-activates both codes when selecting memories for guiding behaviour.
    1. Structural Biology and Molecular Biophysics

    Mechanistic signs of double-barreled structure in a fluoride ion channel

    Nicholas B Last, Ludmila Kolmakova-Partensky ... Christopher Miller
    Combining crystal structures with single-pore analysis of Fluc-family fluoride channel mutants demonstrates that Fluc channels have two separate fluoride ion permeation pathways within the channel complex.
    1. Biochemistry and Chemical Biology
    2. Neuroscience

    Phasic oxygen dynamics confounds fast choline-sensitive biosensor signals in the brain of behaving rodents

    Ricardo M Santos, Anton Sirota
    Fast fluctuations of oxygen, linked to behavioral and neural dynamics in vivo, cause phasic transients is amperometry-measured cholinergic signals via the mechanism of non-steady-state enzyme kinetics in the choline-oxidase-based biosensor.
    1. Neuroscience

    Analysis of fast calcium dynamics of honey bee olfactory coding

    Marco Paoli, Antoine Wystrach ... Martin Giurfa
    Fast calcium dynamics of honey bee olfactory projection neurons revealed an unprecedented heterogeneity of response profile dynamics and explained fundamental aspects of olfactory discrimination and associative learning.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Nanobody repertoire generated against the spike protein of ancestral SARS-CoV-2 remains efficacious against the rapidly evolving virus

    Natalia E Ketaren, Fred D Mast ... John D Aitchison
    High-affinity nanobodies targeting the SARS-CoV-2 spike protein demonstrate sustained effectiveness against current and emerging variants, offering a promising avenue for treatment.