A genome editing resource for discovering, in Drosophila, the organization of cellular structure and function by phosphoinositide signaling.

Imaging of energy status and autophagy during neuronal migration revealed that ATP/ADP levels dynamically change during the migratory and stationary phases and that ATP reduction induces autophagy to maintain migration.

Phosphatidylinositol 3-phosphate interacts with PfHsp70-1 and stabilizes the Plasmodium digestive vacuole under febrile temperatures.

The HCMV virion envelope is derived from the host exosome membrane, and exosome machinery and export pathways facilitate virion egress.

Whereas in a paradigmatic structure an SM protein chaperone clamps its client SNARE shut, a second structure demonstrates that an SM protein can also hold its SNARE open to promote assembly.

The lipid kinase VPS34 complexes I and II are both activated by unsaturation of substrate and non-substrate lipids, curvature, electrostatics and polyphosphoinositides, which play roles in localisation and cellular function.

Both protein-protein and protein-lipid interactions are essential for Rab5 symmetry breaking at the early endosome.

Reconstitution of the endosomal Rab cascade reveals that Rab5 binds and activates the Mon1-Ccz1 guanine nucleotide exchange factor, which in turn recruits Rab7 to membranes to drive fusion.

The presynaptic scaffolding protein Bassoon is involved in regulating neurotransmitter release by controlling synaptic vesicle pool size and vesicular protein turnover through increased ubiquitination and Parkin-dependent autophagy.

Vps29 promotes retromer localization in the adult Drosophila brain, engaging Rab7 and TBC1D5, and its loss triggers age-dependent neuronal impairments in endolysosomal trafficking and synaptic transmission.