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    1. Structural Biology and Molecular Biophysics

    Coil-to-α-helix transition at the Nup358-BicD2 interface activates BicD2 for dynein recruitment

    James M Gibson, Heying Cui ... Chunyu Wang
    A cargo recognition α-helix was identified in Nup358, which is required for activation of a dynein-dependent transport pathway that is essential for brain development.
    1. Developmental Biology
    2. Plant Biology

    The Arabidopsis transcription factor ABIG1 relays ABA signaled growth inhibition and drought induced senescence

    Tie Liu, Adam D Longhurst ... M Kathryn Barton
    Arabidopsis plants slow growth and jettison leaves in response to drought via a mechanism that involves the transcription factor ABIG1.
    1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    A novel single alpha-helix DNA-binding domain in CAF-1 promotes gene silencing and DNA damage survival through tetrasome-length DNA selectivity and spacer function

    Ruben Rosas, Rhiannon R Aguilar ... Mair EA Churchill
    The lysine/glutamic acid/arginine region in chromatin assembly factor (CAF-1) forms a long single alpha-helix DNA-binding domain, facilitating the recognition of tetrasome-length DNA and linking functional domains within the CAF-1 architecture for efficient tetrasome assembly after DNA synthesis.
    1. Developmental Biology

    Specification of diverse cell types during early neurogenesis of the mouse cerebellum

    John W Wizeman, Qiuxia Guo ... James YH Li
    Single-cell RNA sequencing resolves inter- and intra-population heterogeneity, identifies rare cell types, and reconstructs specification trajectories during early neurogenesis of the mouse cerebellum.
    1. Neuroscience
    2. Physics of Living Systems

    Continuous odor profile monitoring to study olfactory navigation in small animals

    Kevin S Chen, Rui Wu ... Andrew M Leifer
    To study odor-guided navigation of small animals such as worms and fly larvae, a novel flow chamber and odor sensor array are presented that better characterize the odors that the animal experiences.
    1. Structural Biology and Molecular Biophysics

    Mechanism of the cadherin–catenin F-actin catch bond interaction

    Amy Wang, Alexander R Dunn, William I Weis
    Deleting the first helix of the α-catenin actin-binding domain results in a slip bond interaction between the cadherin–catenin complex and F-actin, such that the binding interaction is stable at low force.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Detecting molecular interactions in live-cell single-molecule imaging with proximity-assisted photoactivation (PAPA)

    Thomas GW Graham, John Joseph Ferrie ... Xavier Darzacq
    Proximity-assisted photoactivation (PAPA) provides a new way to detect protein–protein interactions in single-molecule imaging of live cells.
    1. Physics of Living Systems

    Nuclear export inhibition jumbles epithelial–mesenchymal states and gives rise to migratory disorder in healthy epithelia

    Carly M Krull, Haiyi Li, Amit Pathak
    Nuclear export inhibition reveals concurrent epithelial and mesenchymal phenotypes that cause mechanosensitive disorder in collectively migrating epithelia.
    1. Cell Biology

    Stress-mediated exit to quiescence restricted by increasing persistence in CDK4/6 activation

    Hee Won Yang, Steven D Cappell ... Tobias Meyer
    Application of a live-cell reporter for CDK4/6 activity reveals rapid activity changes and reversibility in the cell-cycle entry signaling program.
    1. Neuroscience

    Circuit organization of the excitatory sensorimotor loop through hand/forelimb S1 and M1

    Naoki Yamawaki, Martinna G Raineri Tapies ... Gordon MG Shepherd
    Cell-type-specific circuit analysis reveals a highly polysynaptic pattern of excitatory feedforward connections along the somatosensory-to-motor loop, from cuneate via VPL thalamus to S1 and M1 cortex and corticospinal neurons.