Extended biochemical and functional analyses in primary neurons show that early neuronal morphogenesis critically relies on cooperation of the actin nucleator Cobl and its ancestor Cobl-like and uncover their cooperative mechanisms at dendritic branch initiation sites.
Single-molecule TIRF microscopy, biochemical assays and mathematical modeling demonstrate how actin-branch nucleation by the Arp2/3 complex is coordinately regulated by two biochemically distinct activators.
Intersectin counterparts in yeast recruit WASP and WIP to endocytic sites to establish a robust multivalent SH3 domain-PRM interaction network which gives actin assembly onset a switch-like behavior in vivo.
Formin-2 controls spatiotemporal polymerisation of actin filaments, a common mechanism used by apicomplexans for effective segregation of essential chloroplast-like organelles called apicoplasts, and additionally for daughter formation in Plasmodium falciparum.
A reconstituted system has been developed that self-organizes into dynamic actin cortices capable of spontaneous polarization, similar to the initial cortical polarization observed in cells during embryogenesis and development.
Quantitative comparison of clathrin-mediated endocytosis in budding and fission yeast identified conserved mechanisms and species-specific adaptations with broad implications that extend from yeast to humans.