Intranasal immunization of inactivated whole cell of some Gram-negative bacteria induces very rapid and efficient protection against bacterial pulmonary by training alveolar macrophage response, which can be harnessed to design rapid-effecting vaccine against multidrug-resistant bacteria infection.

As well as preventing bone loss, bisphosphonate drugs may boost early immune responses in the lung by acting directly on alveolar macrophages that form one of the first lines of defence against infection.

Transcriptomic and bionergetic analysis shows the importance of HIF-1α activation in enabling tissue-resident alveolar macrophages to perform glycolytic metabolism, which prevents their death and attenuates influenza A virus-induced acute lung injury.

The SARS-CoV-2 Spike proteins demonstrate in vivo cellular tropism, ascertain targets within human mononuclear cells, and provide insights into their functional consequences, thereby enhancing comprehension of their impact on humans.

Time-lapse imaging and the modular recreation of host physiology reveal that alveolar epithelial cells, potential permissive infection sites for Mycobacterium tuberculosis, can restrict early bacterial growth via surfactant secretion.

Monocytes display distinct migratory and functional activity compared to dendritic cells and alveolar macrophages in the lungs.

Based on benchmarking various methods and analyzing multiple real scRNA-seq datasets, a computational platform/workflow and a set of tips for best practices are developed for analyzing causal interactions or relationships in single cells.

The experimental methods that are routinely used to disperse mycobacterial aggregates markedly impact macrophage infection outcomes, which should be taken into account to appropriately interpret host-pathogen interactions studies.