The mechanism triggering HBV membrane fusion involves ERp57, a cellular protein disulfide isomerase, and ultimately leads to the exposition of a fusion peptide that was identified in the pre-S1 determinant.
Iwan Zimmermann, Pascal Egloff ... Markus A Seeger
Synthetic single domain antibody libraries and a binder selection cascade encompassing ribosome and phage display enable the selection of conformation-specific binders against previously intractable membrane proteins within three weeks.
Nicolas Coudray, Georgia L Isom ... Damian C Ekiert
Structure of MlaFEDB ABC transporter with substrate bound in the outward-open pocket of MlaE provides mechanistic insights into lipid transport within the Gram-negative bacterial cell envelope.
Development of specific and high-affinity nanobodies to probe the structure of human acid sensing ion channel-1a and as binding modifiers of the toxins PcTx and MitTx1.
The c-terminus of PI3K plays a key role in regulating kinase activity, with c-terminal disease-linked mutations leading to either activation or inhibition, which reveal a path to specific inhibitors.
Matthew L Rook, Abby Williamson ... David M Maclean
The rotation of the β11-12 linker is a crucial control point for acid-sensing ion channel gating and motion of this linker is required for the channel to desensitize.
Escherichia coli is surprisingly tolerant to chromatinization by archaeal histones, suggesting that histones can become established as ubiquitous chromatin proteins without interfering critically with some key DNA-templated processes.
