A masked cryo-EM image classification approach and the structure of an inhibitor-bound complex provide insights into the molecular flexibility of the catalytic subunit of gamma-secretase.

DNA unwinding triggers a conformational change in the RecD subunit of E. coli RecBCD helicase-nuclease that is transferred through the RecC subunit to activate the nuclease domain of the RecB subunit.

Long-term live imaging reveals the dynamic cell behaviors and progenitor cells that underpin the regeneration of adult limbs in the crustacean Parhyale hawaiensis.

The first malacostracan genome sequence will establish the genetically tractable Parhyale hawaiensis as a model organism in this key animal group.

Cryo-EM structure of the mammalian respiratory supercomplex containing complexes I, III and IV shows a functional asymmetry of complex III, providing strong evidence for directed electron flow in the respirasome.

Building on previous work (Wilkinson et al, 2016), it is shown that inhibition of RecBCD-induced DNA break repair can be used as a co-antibacterial strategy with quinolones.

The tripartite drug efflux pump AcrA-AcrB-TolC, representative of a wide group of pumps from Gram-negative bacteria, enters a transport-competent state through long-distance conformational changes that switch the channel from a closed to an open state.

The near-atomic cryoEM pore complex structure of pneumolysin, the main virulence factor of Streptococcus pneumoniae, shows how the individual domains rearrange during the pore formation.

Caenorhabditis elegans require a GPCR to recognize and rapidly escape from toxin-producing Streptomyces at their head or tail.

Local sharpening of cryo-electron microscopy maps using atomic models can increase the interpretability of densities in cases of resolution variation and facilitates model building and refinement.