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    1. Cell Biology
    2. Neuroscience

    KIF2A regulates the development of dentate granule cells and postnatal hippocampal wiring

    Noriko Homma, Ruyun Zhou ... Nobutaka Hirokawa
    Postnatal KIF2A is a key regulator for axon/dendrite determination of developing dentate granule cells, and its loss developed apical axons in dentate molecular layer, broke hippocampal wiring, and induced temporal lobe epilepsy.
    1. Neuroscience

    A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology

    Benjamin FN Campbell, Antje Dittmann ... Shiva K Tyagarajan
    Anti-gephyrin synthetic protein binders were screened and developed to specifically and robustly detect gephyrin protein and gephyrin-interacting protein partners in the mouse brain, generating a useful tool set for neuroscience.
    1. Genetics and Genomics
    2. Cancer Biology

    Functional characterization of all CDKN2A missense variants and comparison to in silico models of pathogenicity

    Hirokazu Kimura, Kamel Lahouel ... Nicholas J Roberts
    Revised
    Reviewed Preprint v2
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    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Reciprocal regulation among TRPV1 channels and phosphoinositide 3-kinase in response to nerve growth factor

    Anastasiia Stratiievska, Sara Nelson ... Sharona E Gordon
    A dual-color TIRFM study reveals a new form of inflammatory regulation, in which a lipid kinase and ion channel reciprocally regulate each other to amplify the response to painful stimuli.
    1. Biochemistry and Chemical Biology

    Lack of activity of recombinant HIF prolyl hydroxylases (PHDs) on reported non-HIF substrates

    Matthew E Cockman, Kerstin Lippl ... Peter J Ratcliffe
    Assays using recombinant HIF prolyl hydroxylases did not support hydroxylation of more than 20 reported non-HIF substrates under conditions where robust HIF hydroxylation was observed.
    1. Structural Biology and Molecular Biophysics

    Regulatory switch at the cytoplasmic interface controls TRPV channel gating

    Lejla Zubcevic, William F Borschel ... Seok-Yong Lee
    C-terminal domain of thermoTRPV channels regulates channel gating at the cytoplasmic interface through a switch-like process.
    1. Neuroscience

    Ca2+ entry through NaV channels generates submillisecond axonal Ca2+ signaling

    Naomi AK Hanemaaijer, Marko A Popovic ... Maarten HP Kole
    High-speed calcium imaging from the axon initial segment shows that sodium channels are in part permeable to calcium ions.
    1. Microbiology and Infectious Disease

    Bacterial symbiont subpopulations have different roles in a deep-sea symbiosis

    Tjorven Hinzke, Manuel Kleiner ... Stephanie Markert
    Physiological differentiation during symbiosis leads to division of labor between smaller and larger cells in an uncultured bacterial tubeworm symbiont population and results in remarkable metabolic diversity and complexity.
    1. Neuroscience

    Specific and comprehensive genetic targeting reveals brain-wide distribution and synaptic input patterns of GABAergic axo-axonic interneurons

    Ricardo Raudales, Gukhan Kim ... Z Josh Huang
    A lineage-based intersectional genetic approach reveals brain-wide distribution and input connectivity of axo-axonic interneuron cells and establishes experimental access to study the circuit function of a ground truth cell type.
    1. Developmental Biology

    Mask family proteins ANKHD1 and ANKRD17 regulate YAP nuclear import and stability

    Clara Sidor, Nerea Borreguero-Munoz ... Barry J Thompson
    The MASK family of proteins regulate the action of the YAP/TAZ family of transcriptional coactivators, key regulators of stem cell proliferation and cancer.