Killing their neighbors allows bacteria to steal genes, including antibiotic resistance genes, which we observed under a microscope, quantified, modeled, and predicted potentially guiding strategies to combat it.
Sequential therapy with only β-lactam antibiotics achieves surprisingly high potency by exploiting both low rates of spontaneous resistance emergence and low rates of spontaneous cross-resistance among the drugs in sequence.
Selection by national-level antibiotic consumption and vaccination trends drives atypical recombinations, frequently resulting in interspecies sequence exchange at many sites across a bacterial species’ chromosome.
Selection imposed by antibiotics may dominate evolutionary forces acting on opportunistic pathogens like Acinetobacter baumannii, yet chance effects and a prior history in biofilm may constrain resistance and impose collateral sensitivities.
High-throughput droplet-based cultivation of gut microbes reduces biases of traditional cultivation strategies and thereby enables detection of difficult-to-culture organisms, which is required in applications such as antibiotic screening.