The central RNA interference protein, human Argonaute-2, releases its target following phosphorylation on a critical loop in the protein, freeing microRNA-bound Argonaute-2 to seek out and repress additional targets.

The Bi-Genomic Mitochondrial-Split-GFP, where both fragments of the Split-GFP are expressed by separated translation machineries, shuts off cytosolic fluorescence of dual-localized proteins, allowing visualization of their mitochondrial echoforms.

Affinity measurements of microRNA sites representing >1000 different pairing architectures reveal that two binding modes can mediate pairing to the microRNA 3′ region, and that microRNA G and oligo(G/C) residues are most impactful.

HDAC SUMOylation promotes the association of other chromatin remodeling factors with the nuclear Argonaute WAGO-9, which is required for de novo heterochromatin silencing.

SIMR-1 acts downstream of the piRNA pathway to promote siRNA amplification by the Mutator complex and localizes to perinuclear foci distinct from Mutator foci, P granules and Z granules.

The olfactory learning of Caenorhabditis elegans shows that the exonuclease and helicase domains of human Werner syndrome protein (WRN) coordinately regulate the biogenesis of small interfering RNAs to promote neuronal plasticity.

Experimental results and computational modeling reveal many factors that influence if a gene can be efficiently silenced using RNA interference.

Argonaute proteins directly contact adenosine nucleotides in some miRNA target sites, leading to enhanced repression of the target mRNA.

The cooperative antiviral activity of two distinct AGO proteins could be exploited to provide a new strategy for controlling viral diseases in rice.

Genetic and biochemical analyses reveal that two stem-cell-specific microRNAs control stem cell fate decisions between pluripotency and differentiation through repressing Ago2, a key component of the microRNA machinery.