Deep imaging, machine-learning-based segmentation, and tissue annotation resulted in a developmental series of 3D digital ovules with cellular resolution allowing next-level analysis of the ontogenesis of this complex organ.
Building on previous work (Meigh et al., 2013) we show that A88V, a mutation in connexin26 (Cx26) that causes Keratitis-Ichthyosis-Deafness (KID) syndrome in humans, is linked to a lack of sensitivity to CO2 by Cx26 hemichannels.
Live imaging and genetic analyses revealed that notochord vacuoles play a critical role in spine morphogenesis by absorbing vertebral bone growth, thus implicating notochord mechanics in congenital scoliosis.
Application of laser-capture microdissection to planarian intestinal tissue provides a new tool for analysis of tissue-specific gene expression in flatworms, and a new resource to advance investigations of gastrointestinal regeneration.
Interspecies comparison of transcription factor occupancy during embryogenesis reveals potential co-operative relationships between factors and uncovers the inherent plasticity of developmental enhancers to overcome divergence in transcription factor occupancy.