3,754 results found
    1. Biochemistry and Chemical Biology

    An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast

    Jackie Lang et al.
    An assay to assemble kinetochores de novo was developed and used to identify the role of the CENP-T pathway.
    1. Structural Biology and Molecular Biophysics
    2. Neuroscience

    Autoinhibition of Munc18-1 modulates synaptobrevin binding and helps to enable Munc13-dependent regulation of membrane fusion

    Ewa Sitarska et al.
    Biophysical and functional data strongly support the notion that Munc18-1 acts as a template to assemble the neuronal SNARE complex, and that inhibition of this activity underlies diverse forms of regulation of neurotransmitter release.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structural insights into the assembly and polyA signal recognition mechanism of the human CPSF complex

    Marcello Clerici et al.
    Structural, biochemical, and proteomic analyses of a four-subunit core module of the cleavage and polyadenylation specificity factor complex reveal its molecular architecture and specific determinants of polyadenylation signal recognition in human mRNAs.
    1. Microbiology and Infectious Disease

    NSF-mediated disassembly of on- and off-pathway SNARE complexes and inhibition by complexin

    Ucheor B Choi et al.
    NSF is part of a membrane trafficking quality control system that disassembles both properly formed and off-pathway SNARE complexes, and the disassembly activity may be regulated by complexin.
    1. Cancer Biology
    2. Immunology and Inflammation

    Domain-swapped T cell receptors improve the safety of TCR gene therapy

    Michael T Bethune et al.
    Engineered T cell receptors enable targeted immunotherapy with a reduced risk of harmful autoimmunity.
    1. Immunology and Inflammation

    The innate immune sensor IFI16 recognizes foreign DNA in the nucleus by scanning along the duplex

    Sarah A Stratmann et al.
    The scanning-assisted clustering mechanism of IFI16 not only allows efficient assembly on exposed foreign-dsDNA, but also suppresses the engagement of chromatinized self-dsDNA.
    1. Structural Biology and Molecular Biophysics
    2. Neuroscience

    Re-examining how complexin inhibits neurotransmitter release

    Thorsten Trimbuch et al.
    Challenging a widespread model, biophysical and electrophysiological experiments suggest a new mechanism whereby complexins inhibit neurotransmitter release through electrostatic repulsion between their accessory helix and the membranes.
    1. Cell Biology
    2. Physics of Living Systems

    The transition state and regulation of γ-TuRC-mediated microtubule nucleation revealed by single molecule microscopy

    Akanksha Thawani et al.
    Four αβ-tubulin dimers associate laterally on γ-TuRC to define the rate-limiting transition state for microtubule nucleation.
    1. Structural Biology and Molecular Biophysics

    A complex IRES at the 5'-UTR of a viral mRNA assembles a functional 48S complex via an uAUG intermediate

    Ritam Neupane et al.
    The ribosome bound structure of a new IRES reveals novel architecture features used by viruses to hijack cellular translation.
    1. Cell Biology

    Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes

    Aidan M Fenix et al.
    Sarcomeres in heart muscle cells come from stress fiber-like structures that are mechanistically distinct from stress fibers in non-muscle cells.

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