Computational modeling and a cell cycle-reporting axolotl reveal how spinal cord regeneration can be achieved by a signal that propagates 828 μm from the injury site during the first 85 hours post-amputation.

Upon injury, the regeneration of the adult axolotl brain rebuilds neuronal diversity, but alters the original long-distance circuitry and tissue architecture.

Building on previous work (Rodrigo Albors et al., 2015), we assess the contribution of individual cellular mechanisms in the context of spinal cord regeneration in the axolotl.

During axolotl spinal cord regeneration adult neural stem cells reactivate an embryonic neuroepithelial cell-like gene program that implements planar cell polarity to orient cell divisions, coupling polarized spinal cord growth with stem cell self-renewal.

A novel CRISPR-based genetic screen of candidate regeneration genes in haploid axolotl limbs reveals two genes required for proper regeneration.

Although many Apical Ectodermal Ridge signaling components are found in the axolotl limb epidermis, responsiveness to canonical Wnt signaling has shifted to the limb mesenchyme where it regulates mesenchymal Fgf8 expression.

In vivo evaluation of mineralized skeleton in the regenerating axolotl limb reveals a significant osteoclast-driven resorption as an early event with long-lasting impact for tissue integration.

Signaling from the limb nerves regulates the rate of growth and the overall size of the regenerating limb.

Experimental manipulation of a core DNA damage response factor and cell-cycle checkpoint regulators reveals a key role for these processes in the progenitor cells that fuel limb regeneration.

CRISPR-based lineage tracing in the axolotl shows that regenerated limbs are composed of the same cell lineages in the same frequencies as those that gave rise to the original limb.