Deep imaging, machine-learning-based segmentation, and tissue annotation resulted in a developmental series of 3D digital ovules with cellular resolution allowing next-level analysis of the ontogenesis of this complex organ.
Sustained TNF-α induction consolidates transcriptional memory for faster, stronger, and more sensitive subsequent induction in an active DNA demethylation-dependent manner for memory genes including CALCB, a therapeutic target for migraine.
Yeast promoters can harbor multiple natural DNA variants that influence gene expression, interact genetically, evolve under negative selection, alter transcription factor motifs, and remain challenging to predict.
A high-throughput functional genomics approach combining inducible CRISPR-interference and quantitative imaging yields an atlas of 'phenoprints' to guide gene function assignments, identify metabolic pathway-specific morphotypes, and inform antibiotic mechanism-of-action studies.
Transposable elements are not reactivated in natural hybrids of the yeast Saccharomyces paradoxus, but their accumulation is genotype-specific and is not predicted by the evolutionary divergence between a hybrid's parents.