Barcoded rabies virus tracing combined with single-cell RNAseq and in situ sequencing achieves high-throughput retrograde projection mapping and synaptic connectivity mapping at cellular resolution.

A multiplexed retrograde virus tracing method captures single-neuron projectome and transcriptome simultaneously.

A discrete group of interconnected neurons are shown to drive aggressive social interactions in Drosophila females and genetic tools to manipulate these neuronal cell types are provided.

Transcriptomes of cells in the Drosophila visual system combined with anatomy and other information reveal new functional insight into the visual circuit.

Single-cell RNA-sequencing and neural circuitry analyses reveal that sensorimotor transformation of different behaviors which are performed by separate circuit modules in the superior colliculus are molecularly defined by distinct transcriptomic codes of specific neuron subtypes.

Sequencing mRNA from thousands of single cells from the Drosophila brain highlights the extent of cellular diversity and reveals co-expression of specific neuropeptides with particular fast-acting neurotransmitters and monoamines.

Single cell RNA–sequencing and neuroanatomical methods reveal unexpected molecular diversity and highly segregated spatial organization of neuronal cell types within the mouse ventral posterior hypothalamus, including the mammillary nuclei.