Profiling chromatin accessibility and nuclear transcription across Caenorhabditis elegans development and ageing generated the first map of transcriptional regulatory elements and their activities across an animal's life.
Maria I Lazaro-Pena, Adam B Cornwell ... Andrew V Samuelson
The transcriptional cofactor HPK-1 (homeodomain-interacting protein kinase) functions as a key regulator of multiple proteostatic stress responses, each originating from discrete neuronal subtypes within the Caenorhabditis elegans nervous system to preserve neuronal health and maintain organismal proteostasis during normal aging.
The molecular mechanisms beyond the bidirectionality (tTLP and tLTD) of endocanabinoid spike-timing dependent synaptic plasticity have been identified.
Mutagenizing sequences enriched with promoter motifs creates gene expression de novo, and helps to understand promoter logic, regulatory evolution, and the birth of de novo genes.
Desislava P Staneva, Stefan Bresson ... Robin C Allshire
The SPARC complex directs accurate RNAPII positioning for transcription initiation and, in some instances, ensures correct transcription directionality in Trypanosoma brucei.
Yoshitaka Kawasoe, Toshiki Tsurimoto ... Tatsuro S Takahashi
Biochemical analysis in Xenopus egg extracts reveals that the MutSα mismatch sensor retains the DNA-bound replication clamp to maintain a post-replicative temporal window permissive to strand-specific repair of mismatches.
A sophisticated classical mutagenesis assay with next-generation sequencing technology presents a benefit to future research on mutagenesis, DNA repair, and cancer.
Olivier Thouvenin, Ludovic Keiser ... Claire Wyart
The combination of data and modelling shows the mechanisms underlying bidirectional flow of cerebrospinal fluid and its impact on long range transport in the CSF between brain and spinal cord.
