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3,143 results found
    1. Structural Biology and Molecular Biophysics

    Contacts-based prediction of binding affinity in protein–protein complexes

    Anna Vangone, Alexandre MJJ Bonvin
    The number of contacts at the interface of a protein–protein complex, together with the properties of the surface, provides a simple, but well-performing predictor of binding affinity.
    1. Neuroscience

    A low affinity cis-regulatory BMP response element restricts target gene activation to subsets of Drosophila neurons

    Anthony JE Berndt et al.
    DNA motifs tuned for low affinity binding of BMP-induced pMad/Medea transcription factors function to restrict gene activation to small subsets of the many Drosophila neurons that exhibit active BMP signaling.
    1. Computational and Systems Biology

    Building accurate sequence-to-affinity models from high-throughput in vitro protein-DNA binding data using FeatureREDUCE

    Todd R Riley et al.
    A biophysically principled algorithm can build quantitative models of protein-DNA binding specificity of unprecedented accuracy from a leading type of high-throughput in vitro binding data.
    1. Structural Biology and Molecular Biophysics

    Measuring the sequence-affinity landscape of antibodies with massively parallel titration curves

    Rhys M Adams et al.
    The absolute affinities of thousands of variant antibodies are measured in parallel using a combination of cell sorting and high-throughput DNA sequencing.
    1. Neuroscience

    A high affinity RIM-binding protein/Aplip1 interaction prevents the formation of ectopic axonal active zones

    Matthias Siebert et al.
    High affinity interactions with transport adaptors are important to shield the interaction surfaces of cytomatrix components to block fatal premature oligomerization of active zone proteins during axonal transport.
    1. Biochemistry and Chemical Biology

    Evolutionary and functional insights into the mechanism underlying body-size-related adaptation of mammalian hemoglobin

    Olga Rapp, Ofer Yifrach
    Hemoglobin affinity and cooperativity reveal mechanistic insights in how the relation between physiology and evolutionary variations shape a protein's molecular property.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Phosphorylation-mediated PTEN conformational closure and deactivation revealed with protein semisynthesis

    David Bolduc et al.
    C-terminal phosphorylation of the lipid phosphatase PTEN drives a reduction in membrane affinity and leads to a more compact conformation that involves a C2 domain-tail interaction.
    1. Microbiology and Infectious Disease
    2. Plant Biology

    Fungal effector Ecp6 outcompetes host immune receptor for chitin binding through intrachain LysM dimerization

    Andrea Sánchez-Vallet et al.
    Structural and biochemical analysis of a protein called Ecp6, which is produced by a tomato fungus, reveals how the protein prevents plants from launching an immune response to the chitin in fungal cell walls.
    1. Biochemistry and Chemical Biology

    How to measure and evaluate binding affinities

    Inga Jarmoskaite et al.
    Simple guidelines and a checklist are provided for performing high-quality equilibrium binding measurements.
    1. Developmental Biology
    2. Chromosomes and Gene Expression

    Natural variation in stochastic photoreceptor specification and color preference in Drosophila

    Caitlin Anderson et al.
    Binding site affinity and transcription factor levels are finely tuned in nature to regulate stochastic expression, setting the ratio of alternative photoreceptor fates and determining color preference.

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