404 results found
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Biochemical reconstitution of branching microtubule nucleation

    Raymundo Alfaro-Aco et al.
    The protein TPX2 and the protein complex augmin together recruit the microtubule nucleator γ-TuRC to a pre-existing microtubule where they initiate branching microtubule nucleation.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    A composition-dependent molecular clutch between T cell signaling condensates and actin

    Jonathon A Ditlev et al.
    Compositional changes alter actin binding by phase separated T cell signaling clusters, enabling cluster movement by distinct actin networks.
    1. Biochemistry and Chemical Biology

    Mechanism of cargo-directed Atg8 conjugation during selective autophagy

    Dorotea Fracchiolla et al.
    Autophagic cargo receptors recruit the E3-like enzyme for Atg8 lipid conjugation to the cargo and thereby promote local formation of Atg8-positive autophagosomal membranes.
    1. Biochemistry and Chemical Biology

    RNA-binding proteins distinguish between similar sequence motifs to promote targeted deadenylation by Ccr4-Not

    Michael W Webster et al.
    Biochemical reconstitution of deadenylation and analysis of binding kinetics reveals how RNA-binding proteins select their mRNA targets.
    1. Chromosomes and Gene Expression
    2. Microbiology and Infectious Disease

    ParB spreading on DNA requires cytidine triphosphate in vitro

    Adam SB Jalal et al.
    A biochemical reconstitution shows that the accumulation of Caulobacter crescentus ParB on DNA requires cytidine triphosphate and a closed DNA substrate in vitro.
    1. Biochemistry and Chemical Biology
    2. Cancer Biology

    Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes

    Morayma M Temoche-Diaz et al.
    Biochemical fractionation of vesicle sub-populations and in vitro reconstitution studies reveal that Lupus La protein mediates the selective sorting of miR-122 into extracellular vesicles in vitro and in vivo.
  1. Living Science: The importance of remembering

    Eve Marder
    Creativity in science requires the ability to recall information and data, and will suffer if we rely too much on technology to remember things for us.
    1. Chromosomes and Gene Expression

    Monoubiquitination by the human Fanconi anemia core complex clamps FANCI:FANCD2 on DNA in filamentous arrays

    Winnie Tan et al.
    Biochemical reconstitution experiments uncover the ubiquitin-mediated function of a DNA repair protein, key to the understanding of Fanconi Anemia, a cancer-associated bone marrow failure syndrome.
    1. Cell Biology
    2. Computational and Systems Biology

    DASC, a sensitive classifier for measuring discrete early stages in clathrin-mediated endocytosis

    Xinxin Wang et al.
    A new user-intervention-free classification, using single-fluorescent markers, measures conventionally unmeasureable phenotypes in early stages during clathrin-mediated endocytosis.

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