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    1. Microbiology and Infectious Disease

    Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape

    Catherine Baranowski, Michael A Welsh ... E Hesper Rego
    Polar elongating mycobacteria (Mycobacterium smegmatis) require specific cell wall chemistries, those catalyzed by targets of critical antibiotics, to maintain rod shape at aging sites of the bacillus.
    1. Cell Biology

    G-protein-coupled receptor signaling and polarized actin dynamics drive cell-in-cell invasion

    Vladimir Purvanov, Manuel Holst ... Robert Grosse
    siRNA knockdown experiments reveal in detail the signaling pathway that controls cytoskeletal function during cell-in-cell invasion.
    1. Neuroscience

    A novel image segmentation method based on spatial autocorrelation identifies A-type potassium channel clusters in the thalamus

    Csaba Dávid, Kristóf Giber ... Laszlo Acsady
    The spatial autocorrelation method Local Moran’s I was used for image segmentation to delineate pixel clusters representing congruous objects with fuzzy borders, in this case potassium ion channel clusters.
    1. Neuroscience

    A single exposure to altered auditory feedback causes observable sensorimotor adaptation in speech

    Lana Hantzsch, Benjamin Parrell, Caroline A Niziolek
    Hearing a single auditory error while speaking causes an adaptive change that corrects for that error the next time we say a word, providing empirical support for current models of speech sensorimotor adaptation and aligning speech with other motor domains.
    1. Neuroscience

    Deep learning-based feature extraction for prediction and interpretation of sharp-wave ripples in the rodent hippocampus

    Andrea Navas-Olive, Rodrigo Amaducci ... Liset M de la Prida
    A new method is described to identify sharp-wave ripples from the rodent hippocampus with deep learning techniques, which may help to identify and characterize previously undetected physiological events.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Influenza virus transcription and progeny production are poorly correlated in single cells

    David J Bacsik, Bernadeta Dadonaite ... Jesse D Bloom
    Quantification of both viral transcription and progeny production from single influenza-infected cells shows that the cells that produce the most viral RNA often do not produce the most virions.
    1. Biochemistry and Chemical Biology
    2. Plant Biology

    The induction of pyrenoid synthesis by hyperoxia and its implications for the natural diversity of photosynthetic responses in Chlamydomonas

    Peter Neofotis, Joshua Temple ... David M Kramer
    The algal pyrenoid is formed at high O2 levels, even when CO2 is high, implying that a product of photosynthesis, most likely H2O2 acts as a signal.
    1. Cell Biology
    2. Developmental Biology

    Concerted action of neuroepithelial basal shrinkage and active epithelial migration ensures efficient optic cup morphogenesis

    Jaydeep Sidhaye, Caren Norden
    Cellular and tissue level analysis reveals how different cell behaviors involving dynamics of the basal domain cooperate in space and time to shape an epithelial organ.
    1. Evolutionary Biology
    2. Microbiology and Infectious Disease

    Integrating genotypes and phenotypes improves long-term forecasts of seasonal influenza A/H3N2 evolution

    John Huddleston, John R Barnes ... Trevor Bedford
    The combination of phenotypic measures of antigenic drift and genotypic measures of functional constraint improves the accuracy of long-term seasonal influenza A/H3N2 forecasts.
    1. Structural Biology and Molecular Biophysics

    A modular platform for automated cryo-FIB workflows

    Sven Klumpe, Herman KH Fung ... Julia Mahamid
    SerialFIB is an adaptable open-source software designed for streamlining, advanced development and automation of cryo-focused ion beam-based cellular preparations for in situ cryo-electron tomography.