123 results found
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Mechanisms of opening and closing of the bacterial replicative helicase

    Jillian Chase et al.
    Analysis of the Escherichia coli DnaB helicase•bacteriophage λ helicase loader (λP) complex provides insights into helicase opening, delivery to the origin and ssDNA entry, and closing in preparation for translocation.
    1. Structural Biology and Molecular Biophysics

    Hexameric helicase G40P unwinds DNA in single base pair steps

    Michael Schlierf et al.
    Single-molecule FRET experiments reveal that G40P, a DnaB-like helicase, unwinds double-stranded DNA in single base pair steps and its processivity is enhanced by host primase DnaG.
    1. Chromosomes and Gene Expression
    2. Evolutionary Biology

    Cis-regulatory evolution integrated the Bric-à-brac transcription factors into a novel fruit fly gene regulatory network

    Maxwell J Roeske et al.
    The conserved biochemical activity of the duplicate Bab transcription factors were integrated into the regulatory hierarchy of an evolving gene regulatory network by binding site gains in a target gene's cis-regulatory region.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    A broadly neutralizing macaque monoclonal antibody against the HIV-1 V3-Glycan patch

    Zijun Wang et al.
    Rhesus macaques can develop broadly neutralizing antibodies (bNAbs) against the V3-glycan patch of Env that resembles human V3/N332 bNABs.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Frequent exchange of the DNA polymerase during bacterial chromosome replication

    Thomas R Beattie et al.
    DnaB Helicase is the only stable component of the bacterial replisome as the replicative DNA polymerase frequently exchanges in both leading and lagging strands.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Reprogramming the antigen specificity of B cells using genome-editing technologies

    James E Voss et al.
    Antibody paratopes can be added to the human repertoire by CRISPR-Cas9 editing of B lymphocytes using a strategy that allows for hypermutation and class switching of resulting B cell receptors.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome

    Alessandro Costa et al.
    The Mcm2-7 motor unwinds DNA using an approach distinct from that of superfamily III helicases, and accesses multiple ring configurations and assembly states during the initiation of DNA replication.
    1. Evolutionary Biology

    Evidence for adaptive evolution in the receptor-binding domain of seasonal coronaviruses OC43 and 229e

    Kathryn E Kistler, Trevor Bedford
    Phylogenetic and computational methods reveal that at least two seasonal coronaviruses are evolving adaptively in the region of the viral spike protein exposed to the human humoral immune system.
    1. Structural Biology and Molecular Biophysics

    Routine single particle CryoEM sample and grid characterization by tomography

    Alex J Noble et al.
    Fiducial-less tomography on single particle cryoEM samples reveals that most particles are adsorbed to the air-water interface and allows for researchers to diagnose and solve sample, grid, ice thickness, collection, and processing issues.
    1. Structural Biology and Molecular Biophysics
    2. Immunology and Inflammation

    Structural basis for germline antibody recognition of HIV-1 immunogens

    Louise Scharf et al.
    Structural and computational analyses of germline antibody/HIV-1 gp120 complexes provide both general principles relevant to the unusual evolution of potent CD4-binding site antibodies and guidelines for structure-based immunogen design.

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