1,476 results found
    1. Structural Biology and Molecular Biophysics

    Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas

    Alison B Hickman et al.
    The tetrameric structure of a casposase bound to DNA and its biochemical properties show how a transposase could have evolved to perform CRISPR-Cas spacer acquisition.
    1. Cell Biology
    2. Genetics and Genomics

    Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome

    Martin Wegner et al.
    Cloning-free 3Cs technology is developed for the generation of sequence-bias-free covalently closed circular synthesized (3Cs) CRISPR/Cas gRNA libraries that can interrogate the coding and noncoding human genome.
    1. Biochemistry and Chemical Biology
    2. Immunology and Inflammation

    The allosteric activation of cGAS underpins its dynamic signaling landscape

    Richard M Hooy, Jungsan Sohn
    Rigorous biophysical studies reconcile several conflicting views on how cGAS is activated, and present a new unifying allosteric activation mechanism.
    1. Immunology and Inflammation

    Tight nuclear tethering of cGAS is essential for preventing autoreactivity

    Hannah E Volkman et al.
    Tight nuclear tethering of the cGAS DNA sensor maintains it in its resting state and prevents activation by self-DNA.
    1. Microbiology and Infectious Disease

    A chimeric nuclease substitutes a phage CRISPR-Cas system to provide sequence-specific immunity against subviral parasites

    Zachary K Barth et al.
    Horizontal transfer of a sequence-specific DNA-binding domain allows a virus to destroy its subviral parasite and overcome parasite-mediated restriction.
    1. Microbiology and Infectious Disease
    2. Structural Biology and Molecular Biophysics

    Structures of diverse poxin cGAMP nucleases reveal a widespread role for cGAS-STING evasion in host–pathogen conflict

    James B Eaglesham et al.
    Poxin enzymes are a diverse family of insect and viral 2′3′-cGAMP nucleases, which evolved from self-cleaving RNA virus accessory proteases.
    1. Microbiology and Infectious Disease

    Exploiting CRISPR-Cas to manipulate Enterococcus faecalis populations

    Karthik Hullahalli et al.
    Conflicts between CRISPR-Cas systems and antibiotic resistance plasmids can be exploited to selectively eliminate antibiotic resistance from Enterococcus faecalis populations.
    1. Microbiology and Infectious Disease

    A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity

    Lucy Chou-Zheng, Asma Hatoum-Aslan
    Degradosome-associated nucleases PNPase and RNase J2 are required for type III CRISPR immunity against diverse nucleic acid invaders originating from plasmid and phage.
    1. Microbiology and Infectious Disease

    Type III CRISPR-Cas systems can provide redundancy to counteract viral escape from type I systems

    Sukrit Silas et al.
    Cooperation between evolutionarily disparate CRISPR-Cas modules allows bacteria to counter mutational escape by viruses.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands

    Anca M Farcas et al.
    A protein that can recognize regions of DNA with a high proportion of unmethylated CpG dinucleotides, and then recruit polycomb group proteins to these CpG islands, has been identified.

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