Cryo-EM structure of monomeric human Frizzled5 was determined with a universal fiducial antibody at 3.7 Å overall resolution, which supports a simple Fzd/LRP6 heterodimerization mechanism of canonical Wnt/β-catenin signaling.
Analysis of axial polarity distributions shows that Wnt5a regulates collective cell migration in vivo by stabilizing vinculin at adherens junctions and fine-tuning mechanocoupling between neighbouring cells.
Insights into the roles and mechanisms of two of the miRNAs upregulated in human breast cancer stem cells in at least some breast cancers are presented.
Discs large homologue 1 (Dlg1) activates beta-catenin (i.e., canonical Wnt) signaling in CNS endothelial cells to regulate retinal angiogenesis and the development and maintenance of the blood-brain and blood-retina barriers.
The ligand-binding pocket of the Dishevelled PDZ domain can be occupied by Dishevelled's own highly conserved C-terminus, inducing a closed conformation that is ‘opened’ when Wnt signaling stimulates interaction between Frizzled and Dishevelled.
Mathematical and experimental analyses suggest that despite their complex architectures, multiple metazoan signaling pathways act in physiological contexts as linear signal transmitters.
Kif26b, a new component of the evolutionarily conserved Wnt5a-Ror signaling pathway, is controlled by regulated proteolysis and plays crucial roles in mediating vertebrate embryonic tissue morphogenesis.
A set of ex vivo and in vivo experiments, including genetic ablation and regeneration studies, identify a key regulatory function of SOXF factors in muscle stem cells in mice.
