High-level transposon insertional mutagenesis and a broader spectrum of resistance-conferring mutations for selected carbapenems facilitate the evolution of carbapenem resistance in Klebsiella pneumonia clinical isolates.
Acquisition of antibiotic resistance plasmids induces collateral sensitivity to clinically relevant antibiotics in Escherichia coli, paving the way for targeted 'anti-plasmid' therapies able to preferentially eliminate plasmid-carrying bacteria.
Sequential therapy with only β-lactam antibiotics achieves surprisingly high potency by exploiting both low rates of spontaneous resistance emergence and low rates of spontaneous cross-resistance among the drugs in sequence.
Colonisation with resistant Klebsiella pneumoniae in a Cambodian neonatal unit is driven by person-to-person transmission, transmissibility varies by sequence type, and antibiotic consumption generally increases the risk of acquisition.
Integrons deploy a variety of adaptive strategies including excision, shuffling, and duplication of cassettes that foster rapid bacterial adaptation and resistance evolution while protecting the genomic integrity of the host.
A data-driven within-host model reveals that different antibiotics are associated with divergent effects on antibiotic resistance carriage and abundance in hospitalised patients, with important implications for antibiotic stewardship.
Interventions in feedlots and abattoirs place selective pressure on the beef cattle resistome, which differentially impacts the public health risk of antimicrobial resistance from beef production sources.