4,311 results found
    1. Chromosomes and Gene Expression
    2. Human Biology and Medicine

    Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair

    Natasa Savic et al.
    Linking the DNA repair template to the Cas9 ribonucleoprotein complex enhances homology-directed repair of the induced DNA double strand break.
    1. Chromosomes and Gene Expression

    Nucleosomes impede Cas9 access to DNA in vivo and in vitro

    Max A Horlbeck et al.
    Nucleosomes provide a direct and profound block to the activity of the CRISPR effector protein Cas9, suggesting future sophisticated design rules for CRISPR targeting.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

    Steven Lin et al.
    Building on previous work (Jinek et al., 2013), we report a simple and robust system to achieve high fidelity and high efficiency (30% of homologous recombination) genome engineering by homology-directed repair pathway in human cells using cell cycle synchronization and timed delivery of Cas9 ribonucleoprotein complexes.
    1. Chromosomes and Gene Expression
    2. Human Biology and Medicine

    Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering

    Kunwoo Lee et al.
    The guide RNA and donor DNA of the CRISPR/Cas system tolerate large chemical modifications and can be engineered for enhanced delivery and gene editing.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Nucleosome breathing and remodeling constrain CRISPR-Cas9 function

    R Stefan Isaac et al.
    The inhibitory influence of nucleosomes on CRISPR-Cas9 is mitigated by nucleosomal DNA breathing and chromatin remodeling.
    1. Biochemistry and Chemical Biology

    RNA-dependent RNA targeting by CRISPR-Cas9

    Steven C Strutt et al.
    Divergent Cas9 enzymes direct site-specific single-stranded RNA cleavage, reducing infection by RNA phage in vivo and enabling programmable, PAM-independent repression of gene expression in bacteria.
    1. Stem Cells and Regenerative Medicine
    2. Genetics and Genomics

    Multiplexed genetic engineering of human hematopoietic stem and progenitor cells using CRISPR/Cas9 and AAV6

    Rasmus O Bak et al.
    The CRISPR/Cas9 system can be used with recombinant AAV6 donor delivery to facilitate simultaneous, targeted integration into multiple genetic loci in hematopoietic stem and progrenitor cells.
    1. Developmental Biology
    2. Genetics and Genomics

    CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity

    Xiang-Ru Shannon Xu et al.
    Dissection of a cis-regulatory element (CRM) in its native chromosomal context using CRISPR/Cas9 editing and novel 'Active Genetics' reveals new features of CRM function and insights into how such regulatory elements change during evolution.
    1. Human Biology and Medicine

    A homozygous FANCM mutation underlies a familial case of non-syndromic primary ovarian insufficiency

    Baptiste Fouquet et al.
    Bilallelic mutations of FANCM, a DNA-damage response gene whose heterozygous mutations predispose to breast cancer, are involved in a familial case of Primary Ovarian Insufficiency establishing a link between infertility and cancer.
    1. Microbiology and Infectious Disease

    Exploiting CRISPR-Cas to manipulate Enterococcus faecalis populations

    Karthik Hullahalli et al.
    Conflicts between CRISPR-Cas systems and antibiotic resistance plasmids can be exploited to selectively eliminate antibiotic resistance from Enterococcus faecalis populations.

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