In vitro culture of brain endothelial cells leads to a rapid loss of the blood-brain barrier transcriptional and accessible chromatin landscapes that is resistant to the effects of beta-catenin stabilization.
Micropatterned differentiation of human ESCs generates gastrulation cell types – germ layers, extraembryonic, and primordial germ cells with primate characteristics – that show conserved sorting behaviors when dissociated and reseeded as single-cell mixture.
Single cells from a large heterogeneous population can be identified, isolated and clonally expanded using commonly available microscopy equipment and simple reagents, based solely on visual characteristics.
Application of laser-capture microdissection to planarian intestinal tissue provides a new tool for analysis of tissue-specific gene expression in flatworms, and a new resource to advance investigations of gastrointestinal regeneration.
Asymmetric cell division is linked to cell-specific transcription by handoff of a key developmental regulator from the cytokinetic machinery to the adjacent cell pole where it oligomerizes to become stabilized and activated.
A detailed analysis of protein abundance and phosphorylation changes across mitotic subphases and interphase in asynchronously growing human cells has been enabled by combining FACS with quantitative MS-based proteomics.