Expression of Pitx2c at the onset of gastrulation drives convergence and extension movements in the zebrafish embryo by promoting downstream pathways affecting chemokine signaling, integrin-ECM interactions, and planar cell polarity components.
Analysing Myosin II unipolar planar polarisation with high spatial and temporal resolution during Drosophila axis extension reveals how tissue boundaries drive polarized cell intercalation while limiting cell mixing.
Disrupting extrusion, a process that drives epithelial cell death, leads to increased cell survival, poor barrier function, and enhanced cell invasion and, thereby, promotes tumor initiation and progression.
Seemingly contradictory findings of single-molecule and in vivo experiments on a major mechanism of chromosome organization are reconciled by computationally investigating mechanisms of loop extrusion that are consistent with both.
Emergence of hematopoietic stem cells from an aortic component is a unique phenomenon of cell extrusion, with no equivalent in cell biology, and which is adapted to environmental constraints exerted by endothelial cells and hemodynamics forces.
Translationally active polysomes from mammalian cells/tissues are successfully separated with rapidity, high efficiency and exceptional reproducibility by a SEC-based approach, an accessible alternative to the conventional sucrose density gradient analysis.
Reconstitution of DNA loop extrusion in cellular contexts using Xenopus egg extracts shows that condensin extrudes DNA loops non-symmetrically in metaphase, whereas cohesin extrudes DNA loops symmetrically in interphase.