Disruption of the intracellular vimentin network upon cellular stress results in enhanced cell surface vimentin on human osteoarthritic chondrocytes and might represent a novel membrane-associated marker of stress-induced premature senescence.
Lisa L Kirkemo, Susanna K Elledge ... James A Wells
Enzyme tethering of promiscuous biotinylators to the cell surface allows for more rapid and efficient comparative surface proteomic analysis of cells and extracellular vesicles.
The in vivo modification of the canonical intermediate filament protein vimentin with O-linked beta-N-acetylglucosamine affects its function in filament assembly, cell migration and host-pathogen interactions.
Danny LeBert, Jayne M Squirrell ... Anna Huttenlocher
Live real time imaging and perturbation experiments reveal how vimentin integrates with early damage induced reactive oxygen species to mediate collagen rearrangements and epithelial cell projections during wound repair and regeneration.
Abril Angélica Escamilla-Ayala, Ragna Sannerud ... Wim Annaert
Super-resolution microscopy sets a new strategy to comprehend the membrane organization of γ-secretase at single complex resolution identifying nanodomain associations and its diffusion in situ in the living membrane.
Although intermediate filaments are not widely known to contribute to the morphogenesis of cellular protusions, keratin filaments and cytolinkers are critical for the development of microridge protrusions in zebrafish skin.
Jernej Turnšek, John K Brunson ... Andrew Ellis Allen
An iron-sensitive gene cluster encodes proteins that co-localize with phytotransferrin endosomes and are involved in key intracellular iron transformation and trafficking processes in a model marine diatom.
Genetic and chemical genetic analysis pinpoint defective epithelial cells as the driver for NPHP like phenotypes in Invs mutants, demonstrate genetic interaction between Invs and Ift88 and identify the HDAC inhibitor valproic acid as a suppressor for Invs mutant phenotypes.
Embryonic PI3K-Yap activity regulates apical adhesion and proliferation of neural progenitors lining the lateral ventricular surface, to maintain the smooth, non-folded mouse brain and to prevent developmental hydrocephalus.
