Cloning-free 3Cs technology is developed for the generation of sequence-bias-free covalently closed circular synthesized (3Cs) CRISPR/Cas gRNA libraries that can interrogate the coding and noncoding human genome.
In vitro and in cellulo characterization of oligomerization by the cytoplasmic Wnt effector dishevelled and its partner Axin provide new mechanistic principles for Wnt/β-catenin signaling.
Direct estimation of the Hurst exponent shows that endosomes and lysosomes reside in regimes of persistent and anti-persistent motion with heavy-tailed residence time distributions and motion correlated with endocytic function.
Chromosomal instability of cancer can be quantitatively measured by phylogenetic analysis of 200 tumor cells while using evolutionary principles to account for cellular selection.
Posttranslational modifications of tubulins regulate axonal growth and regeneration by controlling the stabilities of microtubules or modulating their interactions with microtubule-associated proteins.
