Single-cell RNA sequencing resolves inter- and intra-population heterogeneity, identifies rare cell types, and reconstructs specification trajectories during early neurogenesis of the mouse cerebellum.
Building on previous work (Haldipur et al., 2014), we show that many key mechanisms controlling cerebellar development are likely conserved between mouse and human, and validate our mouse model of Dandy-Walker malformation.
A multi-phase wrinkling model accounts for the radial and circumferential tension and differential expansion between a uniformly proliferating outer fluid-like layer and an incompressible core that together drive cerebellar folding.
Stride-related modulated firing by neurons of the cerebellar nuclei is required for smooth execution of practiced locomotion and persists more easily with synchronous than asynchronous Purkinje-mediated inhibition.
Nf1 is required during early, but not late, cerebellar development to facilitate neuronal lamination, providing a potential therapeutic prevention strategy for NF1-associated developmental abnormalities.