A genetic screen in a unicellular photosynthetic organism uncovers the first essential signaling component in the chloroplast unfolded protein response that relays information from the chloroplast to the nuclear compartment.
Single-cell analysis of the chloroplast redox response to high light and oxidative stress revealed light-dependent heterogeneity, and was linked to cell fate determination within isogenic diatom populations.
An iron-sensitive gene cluster encodes proteins that co-localize with phytotransferrin endosomes and are involved in key intracellular iron transformation and trafficking processes in a model marine diatom.
The regulatory switch from protection to assimilation, which plants use to exploit natural, fluctuating light, involves movement of the enzyme ferredoxin:NADP(H) oxidoreductase between chloroplast membrane complexes.
Serial-Block-Face Scanning Electron Microscopy (SBF-SEM) associated with biomolecular analysis show that chloroplast differentiation proceeds by distinct ‘structure establishment’ and ‘chloroplast proliferation’ phases, each with differential protein and lipid regulation.