OCT4 and SOX2 display partially independent activity to regulate chromatin accessibility, and highly dynamic activity of OCT4 is required throughout the cell cycle to maintain pluripotency enhancer accessibility.
Profiling chromatin accessibility and nuclear transcription across Caenorhabditis elegans development and ageing generated the first map of transcriptional regulatory elements and their activities across an animal's life.
TET methylcytosine oxidases cooperate with B lineage-specific transcription factors to promote immunoglobulin gene rearrangement by depositing 5hmC, facilitating DNA demethylation and increasing chromatin accessibility at enhancers.
Activity-regulated genes in Drosophila neurons differ from the well-characterized situation in mammals, and these genes provided a strategy to construct reporters for monitoring neuronal activity in fly brains.
The chromatin remodeller BRG1 is recruited to pluripotency-associated gene regulatory elements by the pioneer transcription factor OCT4 to support further transcription factor binding and gene regulation.
The chromatin remodeler and tumor suppressor SMARCB1 acts to restrict superenhancer function to direct neural differentiation of embryonic stem cells while repressing bivalent gene activity in the pluripotent state.