TET methylcytosine oxidases cooperate with B lineage-specific transcription factors to promote immunoglobulin gene rearrangement by depositing 5hmC, facilitating DNA demethylation and increasing chromatin accessibility at enhancers.
Activity-regulated genes in Drosophila neurons differ from the well-characterized situation in mammals, and these genes provided a strategy to construct reporters for monitoring neuronal activity in fly brains.
The chromatin remodeller BRG1 is recruited to pluripotency-associated gene regulatory elements by the pioneer transcription factor OCT4 to support further transcription factor binding and gene regulation.
Integrated modeling of sgRNA positioning, chromatin accessibility, and sequence features enables accurate prediction of effective target sites for CRISPR-mediated transcriptional modulation and design of highly active libraries for genome-scale genetic screens.
Advances in techniques for analysing single cells and tissues have inspired an international effort to create comprehensive reference maps of all human cells - the fundamental units of life - as a basis for both understanding human health and diagnosing, monitoring and treating disease.
An integrative genome-wide approach supports a direct and collaborative role of ETS and AP-1 transcription factors in maintaining endothelial cell-specific and anti-inflammatory gene expression programs.