The CAF1 complex binds single histone H3-H4 dimers, and two such complexes associate with extended DNA elements to ensure the deposition of H3-H4 tetramers, the first step in the assembly of nucleosomes.

After DNA replication, nucleosomes are assembled by two histone chaperone complexes each bound to an H3-H4 histone dimer, suggesting the feasibility for a semi-conservative mode of epigenome inheritance.

The lysine/glutamic acid/arginine region in chromatin assembly factor (CAF-1) forms a long single alpha-helix DNA-binding domain, facilitating the recognition of tetrasome-length DNA and linking functional domains within the CAF-1 architecture for efficient tetrasome assembly after DNA synthesis.

Biophysical and biochemical approaches reveal new insights into the architecture of CAF-1 and the unique mechanism by which CAF-1 tetramerizes histones H3/H4.

NET-seq analysis of 41 S. cerevisiae transcription regulatory factors uncovers their differential and opposing effects on Pol II transcription elongation and antisense transcription, revealing that wild-type transcription is balanced by the joint impact of many regulators.

CRISPR/Cas9 engineered locus-specific proteomics leads to the identification of NuRD, CAF-1, and MBD3L2 as regulators of the early embryonic transcription factor DUX4.

The transcription machinery is required for the disassembly of the promoter-proximal H2A.Z nucleosome, contributing to the constitutive histone turnover at yeast promoters.

A comprehensive resource based on analysis of the responses of embryonic ectoderm cells to signals from the 'organizer' (the node) including transcriptional responses and epigenetic changes with fine temporal dynamics, predicted regulatory interactions, and conservation among vertebrates.

HDAC SUMOylation promotes the association of other chromatin remodeling factors with the nuclear Argonaute WAGO-9, which is required for de novo heterochromatin silencing.

PIE-1 engages SUMO to preserve the embryonic germline fate and also to promote the assembly of a MEP-1/Mi-2/HDA-1 chromatin remodeling complex required for inherited Argonaute-mediated gene silencing in the adult hermaphrodite germline.