Systematic splice site mutagenesis and the identification of splicing intermediates provides evidence that the dominant mechanism for the generation of circular RNA in fission yeast proceeds through an exon-containing lariat precursor.
Cloning-free 3Cs technology is developed for the generation of sequence-bias-free covalently closed circular synthesized (3Cs) CRISPR/Cas gRNA libraries that can interrogate the coding and noncoding human genome.
Transcriptome and eCLIP analyses in mouse and human reveal splicing factor proline/glutamine rich (SFPQ) as a conserved and critical guardian of long-intron integrity, splicing, and circular RNA (circRNA) production.
Extensive genetic diversity and novel genome structures in RNA viruses from arthropods shed important new light on the ancestry and evolutionary history of major classes of vertebrate and plant viruses.
A conserved element in the flavivirus genomic 5′ terminus switches its conformation in response to long-range RNA interactions, and thereby regulates the dynamic recruitment of viral replicase for efficient viral RNA replication.