544 results found
    1. Biochemistry and Chemical Biology

    Stepwise assembly of the human replicative polymerase holoenzyme

    Mark Hedglin et al.
    Fluorescence resonance energy transfer has been used to explore the interactions between DNA polymerases, sliding clamps and clamp loaders as DNA is replicated in human cells.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Single-molecule studies contrast ordered DNA replication with stochastic translesion synthesis

    Gengjing Zhao et al.
    Competing DNA polymerases at the DNA sliding clamp are revealed by single-molecule co-localization studies.
    1. Chromosomes and Gene Expression

    The PCNA unloader Elg1 promotes recombination at collapsed replication forks in fission yeast

    Sanjeeta Tamang et al.
    Unloading the polymerase sliding clamp PCNA from DNA by Elg1 promotes recombination at the RTS1 replication fork barrier by limiting Fbh1 and Srs2 activity.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    The elemental mechanism of transcriptional pausing

    Jason Saba et al.
    Biochemical analyses of transcription complexes, including kinetic studies and probes of translocational and conformational states, establish the elemental mechanism of pausing, which underlies regulation of gene expression in all organisms.
    1. Biochemistry and Chemical Biology
    2. Physics of Living Systems

    Optical estimation of absolute membrane potential using fluorescence lifetime imaging

    Julia R Lazzari-Dean et al.
    Fluorescence lifetime imaging microscopy, paired with fluorescent, voltage-sensitive dyes, provides a method for measuring and quantifying membrane potentials of living cells.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    E. coli TraR allosterically regulates transcription initiation by altering RNA polymerase conformation

    James Chen et al.
    Cryo-electron microscopy structures, combined with biochemical experiments, show how the E. coli F element-encoded TraR protein regulates transcription initiation by altering RNA polymerase conformation and conformational heterogeneity.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Complementary biosensors reveal different G-protein signaling modes triggered by GPCRs and non-receptor activators

    Mikel Garcia-Marcos
    Heterotrimeric G-proteins can be switched on not only by G-protein-coupled receptors but also by cytoplasmic proteins, resulting in different signaling mechanisms in cells depending on the specific type of activator.
    1. Structural Biology and Molecular Biophysics
    2. Chromosomes and Gene Expression

    Structure of the active form of human origin recognition complex and its ATPase motor module

    Ante Tocilj et al.
    The human Origin Replication Complex is shaped as a shallow corkscrew in a classic AAA+ organization reminiscent of clamp loader complexes with highly controlled ATPase activity as exemplified by Meier-Gorlin syndrome mutations.
    1. Cell Biology
    2. Neuroscience

    A novel synaptic plasticity rule explains homeostasis of neuromuscular transmission

    Gilles Ouanounou et al.
    Skeletal muscle cells constantly monitor their own activity and that of their partner neuron at synapses, enabling them to provide the neuron with feedback regarding neurotransmitter release.
    1. Cell Biology

    Yeast Eps15-like endocytic protein Pan1p regulates the interaction between endocytic vesicles, endosomes and the actin cytoskeleton

    Junko Y Toshima et al.
    Phosphorylation of the Eps15-like protein Pan1p regulates how endocytic compartments interact with each other and with the actin cytoskeleton.

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