Quantitative comparison of clathrin-mediated endocytosis in budding and fission yeast identified conserved mechanisms and species-specific adaptations with broad implications that extend from yeast to humans.
An experimentally constrained multiscale mathematical model predicts that branched actin networks self-organize at endocytic sites and bend to produce force, which was verified with cryo-electron tomography of intact cells.
The examination of the ultra-structure and in vivo dynamics of endocytosis in plants reveal plants unique actin-independent, clathrin-mediated endocytosis mechanisms to overcome their unique physiological properties.
CRISPR/Cas9 gene-editing was used to generate a novel endogenous pH-sensitive EGF receptor chimera with the unique utility to study receptor endocytosis in a single-cell and high-throughput experimental formats.
Intersectin counterparts in yeast recruit WASP and WIP to endocytic sites to establish a robust multivalent SH3 domain-PRM interaction network which gives actin assembly onset a switch-like behavior in vivo.