The newly developed antibody-free techniques, HepG4-seq for G-quadruplexes and HBD-seq for R-loops, robustly reveal the comprehensive maps of native G-quadruplexes and R-loops, as well as their roles in transcriptional regulation.

Development of a real-time SnRK2 kinase FRET reporter reveals rapid SnRK2 activation by ABA, but not by Methyl-Jasmonate or elevated CO2, while directly demonstrating basal SnRK2 activity in guard cells.

The SiPIP2;7 aquaporin is a functional CO₂ pore that can be used to improve CO₂ diffusion at the interface between the airspace and leaf mesophyll which limits C₄ photosynthesis.

The essential role of presynaptic NMDA receptors for granule cell GABAergic output elucidates the function of reciprocal spines in recurrent and possibly lateral inhibition of mitral cells during olfactory processsing.

First systemic lysosomal proteomes in association with longevity and tissue specificity in C. elegans uncover lysosomal heterogeneity across multiple scales and provide valuable resources for understanding how lysosomes modulate signal transduction, organelle communication, tissue physiology, and organism longevity.

Rapid, label-free, volumetric, and automated assessment of the immunological synapse dynamics is demonstrated by combining optical diffraction tomography and deep-learning-based segmentation, providing a new option for immunological research.

Astrocyte microdomain calcium transients are mediated by TrpML, stimulated by ROS and tyramine, and mediate astrocyte–tracheal interactions in CNS gas exchange.

DeBias is a generic method to decompose and quantify the confounding, global factors and direct interactions of pairwise interacting variables.

The interaction of ImuB with the β sliding clamp is essential for induced mutagenesis in mycobacteria and could be a novel target for new anti-tuberculosis drugs designed to inhibit the emergence of genetic resistance.

Proximity-labeling using engineered biotin ligases TurboID and miniTurbo enables detection of cell-type-specific and low abundance protein complexes and subcellular proteomes in Arabidopsis and other plants.