A high-throughput functional genomics approach combining inducible CRISPR-interference and quantitative imaging yields an atlas of 'phenoprints' to guide gene function assignments, identify metabolic pathway-specific morphotypes, and inform antibiotic mechanism-of-action studies.
Dot1l and its H3K79 methyltransferase activity are required for thermogenesis, and Dot1l is recruited by Zc3h10 to its targets genes to alter chromatin accessibility to activate the thermogenic gene program.
The olfactory learning of Caenorhabditis elegans shows that the exonuclease and helicase domains of human Werner syndrome protein (WRN) coordinately regulate the biogenesis of small interfering RNAs to promote neuronal plasticity.
Integrating gene expression with genetic association data provided insights into the functional relevance of genetic risk for a complex disease, thus implicating folliculin as a putative diabetic retinopathy susceptibility gene.
Changes in pathways of lipid oxidation, glycolysis, and mitochondrial oxidative phosphorylation are common strategies to cope with high-altitude hypoxia, but some changes require longer evolutionary time to arise.