The importance of synchronous Purkinje cell complex spikes for controlling cerebellar output was investigated by simultaneously recording from cerebellar nuclear cells and arrays of Purkinje cells that synapse onto them.
The cryo-electron microscopy structure of an assembly of the WAVE Regulatory Complex and its activator, the Rac GTPase, plus complementary biochemistry and biophysics, reveal a novel activation mechanism involving two distinct Rac binding sites.
An integrated cryoEM and X-ray crystallography study resolves the structural basis for antibody-mediated targeting of the hantavirus fusion glycoprotein and provides insight into the conformational landscape of the hantavirion surface.
Electrophysiological recordings in monkeys reveal that cerebellar complex spikes encode future reward size when reward information is first made available, but not during reward delivery or smooth pursuit eye movement.
Discrete classes of cerebellar Purkinje neurons show distinct changes in synaptic and spiking activity during motor learning, with simple spikes playing a shifting role during acquisition, expression, and maintenance of learned responses.
Global phosphoproteomic analysis in nerve terminal during exocytosis reveals 252 uniquely regulated phosphosites, highlighting complex regulation of active zone proteins at multiple sites and the role of specific kinases/phosphatases.
Seemingly contradictory findings of single-molecule and in vivo experiments on a major mechanism of chromosome organization are reconciled by computationally investigating mechanisms of loop extrusion that are consistent with both.
Sites at which mitochondria contact the endoplasmic reticulum co-localize the replication of mitochondria and their DNA to help ensure that DNA is distributed appropriately between the newly formed organelles in cells.