Uncoupling of dynamin polymerization and GTPase activity can be induced with dynamin, which allows in vivo visualization of its activity at endocytic pits.
A fully synthetic library of humanized single domain antibodies yields in vitro high affinity antibodies usable in cell biology and translational projects.
Synthetic single domain antibody libraries and a binder selection cascade encompassing ribosome and phage display enable the selection of conformation-specific binders against previously intractable membrane proteins within three weeks.
Development of nanobodies against a model pentameric ligand-gated ion channel demonstrates they can be functionally active as negative or positive allosteric modulators and offers opportunities for future drug development.
Structural and functional analysis of a recently discovered non-canonical potassium channel family reveals a unique selectivity filter that is exposed to permeating ions only in the conductive state.
Structures of the non-canonical potassium channel TMEM175 in open and closed states reveal unique mechanisms for channel gating and the selective permeation of K+ ions.
Structural and biochemical data suggest a mechanism for the Synaptojanin1-catalysed reaction and the role of mutations in the onset of associated neurological diseases.
Conserved contacts on cognate ligands trigger an induced fit pathway that confers selective promiscuity to PD-1, a flexible regulatory protein and promising anticancer target.
