A broadly applicable method that faithfully preserves genetically labeled cellular structures for 3D electron microscopy (EM) and correlated light and electron microscopy (CLEM).

High-resolution, large-scale immunohistochemical mouse brain images showing global views of different brain regions, as well as cellular and subcellular details, identified distinct localization of PKA RIβ and RIIβ regulatory subunits that reveal functional differences.

Correlated light and electron microscopy of cortical neurons reveals a local organization of mitochondria near dendritic spines with diverse functional properties.

Methodology to annotate the multiple origins of axonal projections in dense electron microscopy data of mammalian nervous tissue without the need of chemical label conversion is reported.

Rapid, label-free, volumetric, and automated assessment of the immunological synapse dynamics is demonstrated by combining optical diffraction tomography and deep-learning-based segmentation, providing a new option for immunological research.

The augmentation of resin-embedded sample blocks with magnetic resin allows remote actuation of ultrathin sections and deposition at high packing density onto silicon wafers for correlative light and electron microscopy.

Correlating changes in structure and gene expression in cone photoreceptors of mice daily, between birth and eye opening, created a resource that supports research in photoreceptor function, development, transplantation and repair.

Immunohistochemical labeling of antigens deep within mouse brain tissue sections was achieved without the need for lipid permeabilization, thereby preserving tissue ultrastructure and enabling correlative light and electron microscopy studies.

The pressure of fluid in the inner ear is controlled by opening of cellular valves in the endolymphatic sac to allow for regulated transepithelial fluid flow.

In situ X-ray imaging of biological tissue samples in staining solution while they are being processed for electron microscopy reveals novel insights into the diffusion-reaction kinetics, tissue mechanics, and staining quality.