A landmark-based cross-modality alignment method robust to variation in landmark sets is applied to annotate an EM time series of Caenorhabditis elegans embryonic development as a community resource.
Feedforward inhibition generates motion anticipation by selectively decreasing sensitivity to a stimulus as it moves across the latter part of a retinal ganglion cell's receptive field.
Marta Pallotto, Paul V Watkins ... Kevin L Briggman
Automated segmentation of neurons and identification of synapses in electron micrographs is significantly improved by using simple modifications to chemical fixation protocols that preserve extracellular space in the brain.
SerialFIB is an adaptable open-source software designed for streamlining, advanced development and automation of cryo-focused ion beam-based cellular preparations for in situ cryo-electron tomography.
Lillian K Fritz-Laylin, Megan Riel-Mehan ... R Dyche Mullins
Neutrophil pseudopods are composed of multiple sheets that are inherently flat, protrude in three dimensions, and whose assembly correlates with cell turning.
Adam JM Wollman, Sviatlana Shashkova ... Mark C Leake
Transcription factors form clusters independently of the presence of DNA, which regulate target genes as opposed to individual monomers, addressing a longstanding question of how transcription factors can find gene targets so quickly.
The polyQ tract of pathogenic Huntingtin causes aggregation when expanded in Huntington’s disease, but its two flanking domains control its conformational landscape, proteostasis and neurotoxicity.
One minute biotinylation with APEX2 peroxidase in living cells identifies established and new components of mitochondrial and ER membranes; dataset intersection and overexpression screen identifies SYNJ2BP overexpression as inducing mitochondria-rough ER contacts.
