A novel transgenic rat for studying the role of specific corticotropin-releasing factor type-1 receptor-expressing cell populations in physiology and behavior.

Alternative conformations of membrane protein structures can be predicted to high accuracy with AlphaFold2 by reducing the depth of the multiple sequence alignments used for modeling.

Mutagenesis and activity assays in vitro and in vivo identify residues in the tethered agonist of GPR116, as well as those within the extracellular loops, that are critical for mediating the response to the tethered agonist.

The structure provides key insights into hormone recognition and activation of the glucose-dependent insulinotropic polypeptide receptor.

Spatially-resolved transcriptomics and single-nucleus RNA-sequencing are applied to the human locus coeruleus to characterize the gene expression profiles of norepinephrine (NE) neurons and other cell populations in this critical brain region, with all data made publicly available.