358 results found
    1. Structural Biology and Molecular Biophysics

    Fully automated, sequential focused ion beam milling for cryo-electron tomography

    Tobias Zachs et al.
    The automation of cryo-focused ion beam milling enables previously unfeasible cryo-electron tomography projects.
    1. Structural Biology and Molecular Biophysics
    2. Plant Biology

    Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography

    Benjamin D Engel et al.
    The first 3D views of the native algal chloroplast provide new insights into thylakoid biogenesis, photosynthesis, and carbon fixation.
    1. Structural Biology and Molecular Biophysics

    PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

    Sergey Gorelick et al.
    The PIE-scope resolves the limitations in correlative cryo-focused ion beam milling by adding robustness and ease of use to the workflow.
    1. Microbiology and Infectious Disease
    2. Structural Biology and Molecular Biophysics

    The molecular architecture of engulfment during Bacillus subtilis sporulation

    Kanika Khanna et al.
    In situ cryo-electron tomography unveils the molecular sociology of a developing sporangium in Bacillus subtilis, revealing critical information about cell wall remodeling and membrane migration in bacteria.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Molecular mechanism for direct actin force-sensing by α-catenin

    Lin Mei et al.
    Biophysical and structural studies reveal how low piconewton forces across actin enhance binding by the critical cell-cell adhesion protein α-catenin versus its force insensitive homolog vinculin.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Structural principles of SNARE complex recognition by the AAA+ protein NSF

    K Ian White et al.
    Electron-cryomicroscopy structures of the supercomplex of NSF, αSNAP, and neuronal SNAREs in the presence of ATP under non-hydrolyzing conditions at 3.9 Å resolution reveal interactions between the N-terminal residues of SNAP-25 and NSF.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Molecular and topological reorganizations in mitochondrial architecture interplay during Bax-mediated steps of apoptosis

    Nicholas R Ader et al.
    Correlative microscopy and electron cryo-tomography on apoptotic HeLa cells reveal remodeling of outer and inner mitochondrial membranes, Bax cluster ultrastructure and ATP synthase reorganization in ruptured mitochondria.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    The structure of the COPI coat determined within the cell

    Yury S Bykov et al.
    Cryo-electron tomography of COPI vesicles in the Golgi reveals coat structure, disassembly dynamics, cargo binding, and morphological variability.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structure of Vps4 with circular peptides and implications for translocation of two polypeptide chains by AAA+ ATPases

    Han Han et al.
    Structural and biochemical studies indicate that AAA+ ATPase employ a general mechanism to translocate a variety of substrates, including extended polypeptides, hairpins, crosslinked chains, and chains conjugated to other molecules.

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