302 results found
    1. Structural Biology and Molecular Biophysics

    Beam-induced motion correction for sub-megadalton cryo-EM particles

    Sjors HW Scheres
    Building on previous work (Bai et al., 2013), we describe an algorithm that allows cryo-EM structure determination to near-atomic resolution for protein complexes as small as 170 kDa.
    1. Structural Biology and Molecular Biophysics

    All-atom molecular dynamics of the HBV capsid reveals insights into biological function and cryo-EM resolution limits

    Jodi A Hadden et al.
    All-atom molecular dynamics of the HBV capsid supports a role for structural asymmetry in biological function, reveals the potential for triangular pores to mediate cellular signaling, and indicates that capsid flexibility may limit resolution attainable by cryo-EM.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Cryo-EM structure of a late pre-40S ribosomal subunit from Saccharomyces cerevisiae

    André Heuer et al.
    Near atomic resolution of late 40S ribosomal subunit assembly by cryo-EM reveals immature rRNA active sites stabilized by biogenesis factors.
    1. Structural Biology and Molecular Biophysics

    Cryo-EM structures reveal specialization at the myosin VI-actin interface and a mechanism of force sensitivity

    Pinar S Gurel et al.
    Cryo-EM structures of actomyosin VI in multiple nucleotide states reveal a unique actin-myosin interface and a mechanism of force-sensitivity; furthermore, myosin VI remodels F-actin, suggesting a role for actin structural plasticity during force generation.
    1. Structural Biology and Molecular Biophysics

    Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2

    Dari Kimanius et al.
    Using GPUs for the costly computations in cryo-EM enables structure determination in mere days on a single workstation.
    1. Structural Biology and Molecular Biophysics

    Cryo-EM single particle analysis with the Volta phase plate

    Radostin Danev, Wolfgang Baumeister
    The Volta phase plate enables in-focus single particle analysis at near atomic resolutions, which could expand the capabilities of cryo-EM, especially for small, flexible or heterogeneous macromolecular samples.
    1. Structural Biology and Molecular Biophysics

    Microscopy: Cryo-EM enters a new era

    Werner Kühlbrandt
    Insight
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    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Single-protein detection in crowded molecular environments in cryo-EM images

    J Peter Rickgauer et al.
    High-resolution template matching makes small, densely embedded proteins detectable.
    1. Structural Biology and Molecular Biophysics

    Using the Volta phase plate with defocus for cryo-EM single particle analysis

    Radostin Danev et al.
    Building on previous work in cryo-electron microscopy (Entchev et al, 2015), it shown that a combination of the Volta phase plate and a small amount of defocus can simplify the experimental set-up, increase the data acquisition rate and improve resolution.
    1. Structural Biology and Molecular Biophysics

    cryo-EM structures of the E. coli replicative DNA polymerase reveal its dynamic interactions with the DNA sliding clamp, exonuclease and τ

    Rafael Fernandez-Leiro et al.
    Structures of the replicative DNA polymerase Pol IIIα, the DNA sliding clamp, the proofreading exonuclease, and the processivity switch Tau (τ) suggest a mechanism for quick release during lagging strand synthesis.

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