Cyclin-dependentkinase 2 (Cdk2), the master regulator of S phase events during the cell cycle, controls the earliest step in the motile ciliogenesis pathway in quiescent multiciliatedairway epithelial cells.
Quantitative phosphoproteomics defines the substrates for Cyclin A/Cdk1 kinase during early mitosis and follow up studies validate that one identified substrate, MYPT1, influences the stability of k-MT attachments by regulating Plk1.
Human cullin-RING ligases are buffered to a much greater extent than had been previously appreciated, and the roles of ubiquitin chain extension enzymes are far more nuanced at physiological concentrations.
The general principles that underlie the mechanism of action of the COP9-Signalosome, a key regulator of the largest family of ubiquitin ligase enzymes in human cells, have been identified using structural and kinetic techniques.
The poorly characterized BTB-protein SPOPL is required to maintain the function of the late endosomal system and the endocytic adaptor EPS15 is therein targeted by the SPOPL/Cullin-3 ubiquitin ligase complex for degradation.