In vitro culture of brain endothelial cells leads to a rapid loss of the blood-brain barrier transcriptional and accessible chromatin landscapes that is resistant to the effects of beta-catenin stabilization.
Novel brainstem organotypic cultures that generate rhythmic respiratory motor activity reveal the neural networks that control breathing and a new pathway in the hypercapnic response.
The use of tissue culture reduces the chemical modification of plant DNA, and this has lasting effects on gene expression in the plants and their descendants.
Promoter capture Hi-C in human iPSCs and iPSC-derived cardiomyocytes provides a platform to interrogate gene-regulatory dynamics of cardiomyocyte differentiation and directly links thousands of cardiovascular disease risk loci to hundreds of distal target genes.
During natural visual behavior in mice, orienting towards a target is driven by head movements, during which the eyes stabilize and shift the visual input.
A technique called meta3C provides an elegant and integrated approach to metagenomic analysis by allowing the de novo assembly, scaffolding and 3D characterization of unknown genomes from a complex mix of species
Affinity capture of polyribosomes followed by RNAseq(ACAPSeq) is a technique that harnesses massively parallel sequencing to identify protein-protein interactions from any source from which polyribosomes can be purified.
Bonobo groups that share overlapping ranging areas and engage in regular social exchange show "behavioural group identity" of hunting techniques in the absence of local ecological variation.
Human skeletal muscle progenitors and motor neurons self-organize in three-dimensional co-culture to form functional neuromuscular junctions that developmentally mature from the embryonic to the adult state.
