In vitro culture of brain endothelial cells leads to a rapid loss of the blood-brain barrier transcriptional and accessible chromatin landscapes that is resistant to the effects of beta-catenin stabilization.
Single cells from a large heterogeneous population can be identified, isolated and clonally expanded using commonly available microscopy equipment and simple reagents, based solely on visual characteristics.
An in vivo disulfide crosslinking assay shows preferential disassembly of nucleosomes with two H2A.Z histones by transcription machinery in yeast and conjugation to one or two ubiquitin moieties in human cells.
A detailed analysis of protein abundance and phosphorylation changes across mitotic subphases and interphase in asynchronously growing human cells has been enabled by combining FACS with quantitative MS-based proteomics.
Asymmetric cell division is linked to cell-specific transcription by handoff of a key developmental regulator from the cytokinetic machinery to the adjacent cell pole where it oligomerizes to become stabilized and activated.